Flowjo basic tutorial pdf

This tutorial is designed to introduce you to flowjo by demonstrating the analysis of a simple experiment. Great listed sites have flowjo compensation tutorial. Within each revision are listed the new features major and minor, and any bug fixes. The workspace contains a list of all of the data samples that you load, the gates, statistics and other analyses that you apply, and the table and graphical layout that you design. This can be the all samples group or the basic tutorial data group. One is located on the taskbar, and the other is located in the navigate band of the flowjo tab. From the website is also possible to download tutorials containing data sets and pdf files, with a detailed description of the steps to follow in order to perform an analysis. Flowjo basic tutorial getting started 2 of youtube. Tutorials flowjo documentation seqgeq documentation grant resources documents flow cytometry news flowjo africa flowjo and bd are one.

Part 4 of a part run through of the basic tutorial. To begin this tutorial, you first need to install flowjo. The pdf document contains written instructions for the basic tutorial. In contrast to most of the other analysis software packages this is easy to use and comparatively intuitive. Save these files to disk and doubleclick on the compressed file to extract them on a mac and right click to extract them on a pc. Fcs flow cytometric standard files and a pdf of the tutorial. Save these files to disk and doubleclick on the compressed file to. Flowjo university is our hub for free education covering everything from introductory to advanced courses. I want to model the flow of fluid in liquid composite molding using flow 3d software. For a more thorough introduction to basic flowjo usage, download the basic tutorial from the following link. The basic tutorial pdf is excellent and gives you an excellent grounding in the basics of assembling data plots and performing statistical analysis. Since its establishment in 2004, our mission is to provide researchers access to well characterized cytometry equipment for sophisticated cell sorting and cell analysis experiments, as well as services in data analysis.

Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they. You must be connecting from a stanford ip address or using a vpn connection to stanford. Im going double click and call this table cd8 positive basic gates. Save these files to disk and doubleclick on the compressed file to extract them on a mac and rightclick to extract them on a pc. You can access any section directly from the section index available on the left side bar, or begin the tutorial. Flowjo basic tutorial lesson 4 batching in the workspace 7 of by flowjo media. No data is allowed to save in other directory on flowjo computer. Following this link will bring you to a page where you can download our basic tutorial and advanced 8 color tutorial as.

In the same way, the sample files load into the flowjo workspace and appear as part of the all samples group. It would be great if you can suggest me a way to do that. Selecthighlight the basic tutorial data folder, and click choose. Alternatively, you can add samples using the add samples action buttons. Flowsom using selforganizing maps for visualization and interpretation of cytometry data. If i drag and drop those highlighted populations from the workspace gating tree, into the table editor, itll create a list of those gated populations along with the statistic that were going to enumerate in my table here. Part 3 of a part run through of the basic tutorial. Flowjo standard operation protocol basic operation hku. This information can be used to individually sort or separate subpopulations of cells. Flowjo is software for the analysis of flow cytometry data. Download flowjo download seqgeq flowjo exchange plugins learn webinars flowjo university tutorials flowjo documentation seqgeq documentation. Part 2 of a part run through of the basic tutorial. In this video, flowjo application scientist mike stadnisky introduces you to the newest version of flowjo with a 24part run through of the advanced tutorial. Welcome to the gw school of medicine and health sciences smhs flow cytometry core facility website.

This tutorial will introduce you to basic concepts and mechanics of flowjo vx. Selecthighlight the basic tutorial data folder, and. Somebody can recommend me the tutorials pdf video of flow 3d v10. Groups in flowjo 8 of 12 batching and iteration 9 of 12 flowjo v10 plugins and the script editor 10 of 12 an introduction to the discovery tools 11 of 12. The workspace contains a list of all of the data samples that you load, the gates, statistics and.

You are strongly suggested to read pdf files in the folder of flowjo tutorial. There was a basic tutorial first, and then there was an 8 color immunophenotyping tutorial that i also did. Flowjo tutorial coefficient of variation median scribd. Flow newbie looking for tutorials on flowjo analysis. Somebody can recommend me the tutorials pdfvideo of. Flowjo university learn flowjo or seqgeq on your own.

Listed below are the changes made to flowjo since the release of flowjo, version 6. In this video, flowjo application scientist mike stadnisky dives deeper into analyzing data in the newest version of. The changes are listed in reverse chronological order. The basic principle of flow cytometry is the passage of cells in single file in front of a laser so they can be detected, counted and sorted. Part 8 of a part run through of the basic tutorial. The outline of the reference manual is shown below. Introduction there are 6 steps to analyze data by flowjo. Reading through and performing the steps using the provided data and workspace will give you a feel for the workflow through flowjo. It was not the most intuitive program to learn, but i used the tutorials from their website and they helped a lot. Flowjo is the leading analysis platform for singlecell. Flow cytometry flow cytometry is the technical process that allows for the individual measurements of cell fluorescence and light scattering.

This tutorial will quickly introduce you to flowjo using an example experiment. In this video, flowjo application scientist mike stadnisky dives deeper into analyzing data in the newest version of flowjo. The flowjo basic tutorial video is another way to follow along. Current protocols in cytometry online access to through lane library. This tutorial will quickly introduce you to flowjo using an example experiment, where a fitc anticd8 reagent is titrated to determine the most economical concentration for staining in future experiments. Flowjo introduction tutorial free download as pdf file. Workspaces are the basic environment that flowjo uses. This environment is presented in the form of a wo r k s p a c e. Flowjo basic tutorial flowjo software is used for the analysis of flow cytometry data. Cell components are fluorescently labelled and then excited by the laser to emit light at varying wavelengths. Not for use in diagnostic or therapeutic procedures.

The data we are using for this tutorial are from a basic antibody. A fitc anticd8 reagent was titrated to determine the appropriate reagent concentration. Somebody can recommend me the tutorials pdfvideo of flow. This tutorial is designed to introduce you to the basics of the program. Best alternative software for doing flow cytometry. The purpose of this user guide is to explain the acquisition module of flowjo 7.

Using the file browser, navigate to the basic tutorial data folder. The tutorial data is a 9color panel designed to interrogate phenotypic and functional responses of human t cells. You are strongly suggested to read pdf files in the folder of flowjo tutorial and watch videos in the folder of flowjo tutorial video on desktop before you start to use flowjo to analyze data. This process is performed at rates of thousands of cells per second. Note that there are also options for batching directly to a printer, pdf or. Following this link will bring you to a page where you can download our basic tutorial. The properties measured include a particles relative size, relative granularity or internal complexity, and relative. Flowjo tutorial flowjo is a software application designed to create an integrated environment for viewing and analyzing flow cytometric data. Introduction flowjo basic tutorial flowjo is an integrated environment for viewing and analyzing flow cytometric data, presented in the form of a workspace. Flowjo collectorsedition fjce iscytekbranded acquisition software blending tree star flowjo workspace features and data acquisition for cytek modified cytometers. Flowjo basic tutorial flowjo is software for the analysis of flow cytometry data. The fluorescence can then be measured to determine the amount and type of. The tutorial is divided in 6 parts and each part is divided on its turn into different sections covering a topic each one.